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Slides: Enabling Powerful Innate Defenses Against HIV
Slide 1.
Enabling Powerful Innate Defenses Against HIV
Slide 1.
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Genomic Structure of HIV-1
Slide 2.
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Genomic Structure of HIV-1
Slide 3.
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Worldwide HIV Infection: A Snapshot
Slide 4.
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Worldwide HIV Infection: A Snapshot
Slide 5.
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Worldwide HIV Infection: A Snapshot
Slide 6.
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Worldwide HIV Infection: A Snapshot
Slide 7.
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Worldwide HIV Infection: A Snapshot
Slide 8.
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Worldwide HIV Infection: A Snapshot
Slide 9.
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Worldwide HIV Infection: A Snapshot
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The Global HIV Epidemic
Slide 11.
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The Global HIV Epidemic
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HIV-1 Life Cycle
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HIV-1 Life Cycle
Slide 14.
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HIV-1 Life Cycle
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HIV-1 Life Cycle
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Slide 17
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Slide 18
Slide 18.
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HIV-1 Life Cycle
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HIV-1 Fusion
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HIV-1 Fusion
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HIV-1 Life Cycle
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HIV Factoids
Slide 23.
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HIV-1 Life Cycle
Slide 24.
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Post-integration HIV Latency
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Slide 26
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Slide 27
Slide 27.
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Exciting New HIV Drug Targets
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Slide 29
Slide 29.
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APOBEC3G
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Slide 31
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Slide 32
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Slide 33
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Slide 34
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Slide 35
Slide 35.
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HIV-1 Vif
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How does Vif counter?
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Kim Stopak, J.D.
Slide 38.
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HIV-1 WT infection
Slide 39.
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HIV-1 WT infection
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An Exciting New Target
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Strategy For Identifying Small Molecule
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Why is Cellular APOBEC3G Unable to Inhibit Wild Type HIV-1 Infection?
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Slide 44
Slide 44.
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Ya-Lin Chiu, Ph.D
Slide 45.
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Strategy
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Endogenous APOBEC3G
Slide 47.
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Endogenous APOBEC3G
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Slide 49
Slide 49.
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Vanessa Soros, Ph.D
Slide 50.
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APOBEC3G enzymatic activity
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Recruitment of A3G
Slide 52.
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A 20-year riddle in HIV biology
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HIV Encounters
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What form of APOBEC3G is present in resting CD4 T-cells?
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PHA/IL-2 Stimulation
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PHA/IL-2 Stimulation
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Defferentiation of Monocytes Promotes
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Defferentiation of Monocytes Promotes
Slide 59.
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HIV Encounters
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Slide 61
Slide 61.
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Experimental Strategy
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Construction of Experimental
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Knockdown of Exogenous HA-APOBEC3G
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Jason Kreisberg BMS
Slide 65.
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Time Course of siRNA-mediated
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Slide 67
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Single-cycle Infection Assay
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siRNA Knockdown
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siRNA Knockdown
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siRNA Knockdown
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Summary
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Slide 73
Slide 73.
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Significance
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LMW APOBEC3G
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Recruitment of APOBEC3G Into HMW
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Vif Target HMW
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Therapaeutic Implications
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Acknowledgements
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Acknowledgements
Slide 80.
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Thanks to the Funders of These Studies
Slide 81.
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